Journal: EMBO reports
Article Title: Modulating tumor immunity using advanced microbiome therapeutics producing an indole metabolite.
doi: 10.1038/s44319-025-00386-9
Figure Lengend Snippet: Figure 2. EcN-mediated delivery of IAA reduces tumor burden in a colorectal syngeneic tumor model.
Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models CT26 murine colorectal carcinoma cell line ATCC CRL-2638 MC38 murine colorectal carcinoma cell line Professor Janine Erler, Copenhagen University HT29-Lucia AhR reporter cells InvivoGen ht2l-ahr BALB/c mice Janvier C57BL/6 mice Taconic Recombinant DNA Cas9 plasmid (pHM-156) This study gRNA plasmid (pMB-TnaA) This study Antibodies FOXP3 Abcam ab215206 ELA2 Neutrophil Elastase Abcam ab315901 CD68 Abcam ab283654 Granzyme B Abcam ab255598 CD4 Abcam ab183685 CD8 Cell Signaling D4W2Z CD45-BUV395 Clone 30-F11 BD Biosciences #564279 IFNγ-BV421 Clone XMG1.2 BD Biosciences #563376 CD4-FITC Clone RM4-5 BD Biosciences #553047 GzmB-PE Clone GB11 BD Biosciences #561142 CD3-PECF594 BD Biosciences #562332 CD8-APC Clone 53-6.7 BD Biosciences #553035 Dead cells – eFluor 780 Thermo Fisher Scientific #65-0865-18 Chemicals, enzymes, and other reagents Q5 High-Fidelity DNA Polymerase New England Biolabs M0541S Gibson Assembly Kit New England Biolabs E5510 One ShotTM TOP10 Chemically Competent E. coli Thermo Fisher Scientific C4040-10 NucleoSpin Plasmid EasyPure Macherey Nagel 740727.250 Collagenase IV Sigma-Aldrich 17104019 DNase I Sigma-Aldrich 11284932001 TrypLE Express Thermo Fisher Scientific 12604013 Zeocin InvivoGen ant-zn-05 Fetal bovine serum Thermo Fisher Scientific A5670701 Penicillin–streptomycin Thermo Fisher Scientific 15140148 QUANTI-LucTM Luciferase Detection Reagent InvivoGen rep-qlc4r2 RPMI1640 Thermo Fisher Scientific 11875093 Software Version RStudio CRAN 4.1.0 Reagent/resource Reference or source Identifier or catalog number FlowJo® BD Biosciences 10.10 Other Synergy H1 plate reader BioTek GentleMACS C Tubes Miltenyi Biotec 130-093-237
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